High temperature disrupts the structure of proteins and certain chemicals such as acetone and methanol dissolve the phospholipids, leading to damage of the membrane and therefore leads to increased permeability. Cells were incubated in the presence of methanol and acetone at the indicated concentrations.
Bottles containing solvents must never be emptied. Safety glasses must also be worn throughout the lab to protect the eyes from chemicals.
All of the other items go into separate white buckets. General Aspects Gloves must be worn at all times to protect from pigment staining.
The used pasteurs and cuvettes go in the pots on the bench. The higher the temperature, the more betacyanin is lost from the vacuole. Lab coats must be fully fastened to protect clothes from staining.
The data is shown in the graph below. After the 45 minute period, pigment leakage was recorded using a spectrophotometer set at nm.
The data above shows that acetone showed more damage as measured by betacyanin release than methanol. Get Full Essay Get access to this section to get all help you need with your essay Cell membrane report educational issues.
However when we increase the temperature the relatively weak forces holding the different parts of the polypeptide chains together like hydrogen bonds can be disrupted very easily- this damages the vacuole and makes holes in the Cell membrane report membrane, inducing leakage. The bottles were left to incubate for 20 minutes and then the beetroot cylinders were removed.
When cells are cut, the cell membranes are mechanically ruptured. This would only have had a small effect on my results, because the vacuole of the beetroot would have only released slightly more betacyanin, as the molecules would have only had a very small amount more heat energy to convert into kinetic energy.
Additionally, the highest concentration of each solvent showed the most damage. Practical Skills in Biomolecular Sciences, 4th Ed. This would have had a slight effect on my results because the rate of diffusion of betacyanin particles across the plasma membrane is increased, as the surface area of the beetroot increases.
Following incubation, damage to cell membrane integrity was assessed by quantifying leakage of the pigment betacyanin via measuring light absorbance at nm. The vacuole is enclosed by a single membrane called tonoplast and the whole cell is enclosed by cell membrane made up of phospholipids. Disposal of Material and Equipment All gloves and pipette sleeves must be disposed of in the blue waste bins.
Beetroot contains a red pigment called betacyanin, which is located in the large central vacuole of the beetroot cells. The darker the sample, the greater the absorbance. After the 45 minute period, the beetroot cylinders were removed from the distilled water and pigment leakage was recorded using a spectrophotometer set at nm.
Get Access Membrane Lab Report Essay Sample The cell membrane consists of mostly phospholipids and proteins which gives the cell its selectively permeable nature.
The aim of this experiment is to observe the effect of physical and chemical stress on the membrane permeability of beetroot. However the sources of error and limitations in my results may have made my results slightly less accurate, but other students in my class found the same patterns occurring and roughly the same results which would vary slightly between each beetroot anyway.
I can firmly conclude that there are no apparent anomalies in my results and none of my sources of error or limitations of my experiment are enough to deem my results unreliable. As long as the cells and their membranes are intact, the pigment will remain inside the vacuoles.
More cells being damaged will cause a higher red colour intensity in the solution.
All of the beetroot cylinders were washed under running tap water for a duration of 5 minutes until no more colour appeared in the wash water or on the surface. They can also cause damage to skin permeability barrier. Chemical Hazards The solvents acetone and methanol should not be inhaled as it can cause damage to mucous membranes.
Distilled water was used as the blank. Another limitation was cutting the beetroot into pieces. However, if the membranes are damaged, betacyanin will leak out and produce a red colour in the surrounding water. Potential injury may arise from razor blades and cork borers.
After heating, all the cylinders were placed into a boiling tube containing 15ml of distilled water and were then allowed to stand for 45 minutes. It would also have been better to have had the time to repeat each temperature more times to make the results more reliable and so I could be sure the results were not gained through chance.
The function and permeability of the cell membrane depends on its whole structure.tonicity on cell membrane lab report Essay Words | 9 Pages Effects of Tonicity on Cell Membrane Abstract The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C).
1 BioLab 3 Cell Membranes Lab Report Student Name: Kendall Austin I. Diffusion Define the following terms. Solvent Any substance capable of dissolving another substance. Solute Any substance dispersed by water Solution.
Membrane Lab Report Essay Sample The cell membrane consists of mostly phospholipids and proteins which gives the cell its selectively permeable nature. The function and permeability of the cell membrane depends on its whole structure. The plasma membrane’s structure is composed in such a way that it can discriminate as to which substances can pass into the cell.
This enables nutrients to enter the cell, while keeping unwanted substances out. For example. it will dissolve the cell membrane as cell membrane is made up of lipid.
we can use alcohol as a substance to investigate the effect of it to the plasma membrane. the leakage of the red-coloured-dye from beetroot pieces into the surrounding.
A cell membrane, plasma membrane or plasmalemma is a selectively permeable lipid bilayer coated by proteins which comprises the outer layer of a cell. The plasma membrane works between the.Download