Imagine that you repeated the experiment in Part 1 and examined the results the next day. Why do you think you got the results you did?
The results of this test confirm the same by the slope of each line and thereby Peroxidase lab report are able to reject our hypothesis that temperature has no effect on peroxidase rate of reaction.
In accordance with these properties, we will see how certain factors affect the reaction rate of peroxidase. If you did not have ampicillin to select for transformed colonies, given what you know about the pUC8 plasmid and JM strain, is there another way you could determine transformation efficiency?
To do this, we tested effects on peroxidase activity, first, with different amounts of the enzyme, next at temperatures of 4oC, Room Temperature, 32oC, 48oC and boiling; then, at pH 3, pH 5, pH 7 and pH 9; and, finally, with the competitive inhibitor, hydroxylamine.
A similar result was found when hydroxylamine was added to the peroxidase and it caused an inhibition reaction. It is known that when heat is applied to molecules, they move faster and collide more as the temperature rises. Do the same for the Luria agar plates with ampicillin. Remove the tubes from the 42 degrees Celsius waterbath and immediately place on ice for two minutes.
The experiment was messed up due to the prolonged exposure to the cold temperatures which killed some of the cells. Gently tap the tube with your finger to mix the solution. Dip the loop into the DNA stock tube, when you remove the loop from the solution check to be sure that there is a drop of liquid contained in the loop area.
One would also be careful not to contaminate the pipets, loops, or petri dishes. There was a large amount of growth on the non-plasmid treated cells. To remove the bacteria from the transfer loop, and break them apart, place the loop into the calcium chloride and twirl rapidly.
One might also accidentally contaminate one of the sterilized pipets or loops by touching to another non-sterilized surface. Once this new product is released, the enzyme can bind again with more of these molecules needing conversion.
Count and record the number of colonies on each plate. As for pH effects on peroxidase activity, Graph 3, indicates that the amount of acidity or basicness to a solution changes the three-dimensional structure of the enzyme and thereby changes the ability to bind with the substrate in an effective manner.
We developed several null hypotheses for these experiments: Explain what may or may not have occurred. Conversely, it was a challenge to get accurate absorbance readings at 2.
Here we tested the null hypothesis: The hypothesis might have been correct if the experiment had not have gone wrong early on in the experiment. This test was important so that we could ascertain the best amount of concentration to use in the subsequent experiments with the spectrophotometer set at absorbance nm and timed recordings at second intervals for a total of 2 minutes.
Be sure to label all four plates with your group name. In order to follow the rate of reaction for the breakdown of hydrogen peroxide, we used guaiacol, a colorless dye, which donates electrons and turns brown when it is oxidized.
One of the errors of this experiment was that one waited too long to complete the experiment after one got the materials. Remove the tubes from the ice bath and add 0. Describe the results observed on each plate: In other cases competitive inhibitors are at work and prevent a substrate from being bound to the active site on the enzyme.
In the case of boiling of the enzyme there was no rate of reaction found whatsoever.
We used this dye so that we could measure the absorbance with the spectrophotometer as the hydrogen peroxide is being broken down and the color change gets stronger over specific time intervals.
Gently tapping the loop against the side of the tube may help dislodge the bacteria. The optimal amount of peroxidase concentration to be used in the subsequent experiments was determined to be 1.
Be sure not to transfer any agar to the tube.The Effect of pH on Enzyme Activity Hypothesis The pH will have an effect on enzyme activity. As seen through the amount of water displaced, the enzymes in the optimal pH environment of 6 will be the most active, displacing the most water, followed by the basic environment of 9, and the acidic.
Lab Report Enzymes Essay Introduction This experiment is designed to test the enzyme activity of Catecholase the enzyme in potato juice, in many different conditions such as varying temperatures, ranging pH, and concentration of the enzyme, as well as concentration of the substrate.
Enzyme (Peroxidase) Concentration And Enzyme Activity (Lab Report Sample) Instructions: Question Description: This is the enzyme lab report, reading and following the word Writing a lab report and you can understand what you are going to do for the report.
PURDUE UNIVERSITY INSTRUMENT VAN PROJECT Factors Affecting Enzyme Activity rate of the reaction. The enzyme which you will investigate in this exercise is peroxidase.
Peroxidase catalyzes the Using the mixing procedure described in the previous lab procedure (procedure 9), quickly mix the seconds and record the data. Lactase Enzyme Lab Report Quickly add 1 mL of lactase to the 2 tubes labeled “Milk + Lactase” and “Sucrose + Lactase, “ and 1 mL of water to the 2 tubes labeled “Milk + Water” and “Sucrose + Water.”.
Dec 06, · Biology 12 Peroxidase Experiment. For Later. save. Related. Info. Embed. Share. Print. Related titles.
Exp 4 Restriction Enzyme. AP Biology Enzyme Lab Report. Lab Determining the Degree of Specifity of the Enzymes Polyphenol Oxidase and Peroxidase.
Determining the Properties of an Enzyme. Peroxidase Lab. Peroxide potato lab 5/5(5).Download